|
| Status |
Public on Apr 30, 2019 |
| Title |
donor 983, omentum, vehicle control tech rep2 |
| Sample type |
RNA |
| |
|
| Source name |
omental adipose tissue explant
|
| Organism |
Homo sapiens |
| Characteristics |
donor: donor 983, female, 33yrs old, BMI=43.0 kg/m2
treatment: vehicle control
depot: omentum
|
| Treatment protocol |
Tissues were treated with vehical control or 1 μM rosiglitazone for 7 days.
|
| Growth protocol |
Omental human adipose tissue fragements were cultured in explants culture system with serum free M199 with 0.7 nM + 10 nM Dexamethasone for 7 days. Tissues were refed every 2 days and one day prior to the harvest on day 7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol followed and cleaned with RNA-easy mini cleanup kits from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the Ambion WT Expression Kit (Life Technologies, Grand Island, NY) according to the manufacturer's protocol, followed by the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the GeneChip Human Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
Gene expression data from omental adipose tissue explant treated with vehicle control.
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1) and an Entrez Gene-specific probeset mapping (version 15.1) from the Molecular and Behavioral Neuroscience Institute (Brainarray) at the University of Michigan.
|
| |
|
| Submission date |
Nov 19, 2018 |
| Last update date |
Apr 30, 2019 |
| Contact name |
Mi-Jeong Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston University
|
| Department |
Medicine
|
| Street address |
650 Albany St 810
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL16522 |
| Series (1) |
| GSE122721 |
Effects of rosiglitazone on human omental adipose tissue gene expression |
|
