|
| Status |
Public on Apr 30, 2019 |
| Title |
metastasis sample from intestinal tumor reinjected specimen - T2 [2343 2016] |
| Sample type |
RNA |
| |
|
| Source name |
metastasis sample from intestinal tumor reinjected specimen - T2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
technical replicate: rep3
tissue: metastasis sample from intestinal tumor reinjected specimen
reinjection: T2
|
| Treatment protocol |
Allografts were dissected out of the adult host and splitted in several pieces. One was introduced in lysys buffer for RNA extraction and the rest were transplanted to new hosts.
|
| Growth protocol |
Ttumours were grown and maintained by serial transplantation into adult hosts at 25ºC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from tumor using magnetic beads. 25 ng of total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2). Amplification was performed for 17 cycles.
|
| Label |
biotin
|
| Label protocol |
8 µg of cDNA was subsequently fragmented and labeled using GeneChip Mapping 250K Nsp Assay Kit (Affymetrix; catalog # 900766), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cDNA was hybridized to the microarray for 16 hours at 45ºC, washing and staining of microarrays was performed using a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
Arrays were scanned with GeneChip scanner GSC3000 (Affymetrix, Santa Clara, CA)
|
| Data processing |
RMA normalization method as implemented in affy Bioconductor R package
|
| |
|
| Submission date |
Jan 18, 2019 |
| Last update date |
May 02, 2019 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
[email protected]
|
| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
|
| Street address |
Parc del Taulí, 1
|
| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE125312 |
Genome wide gene expression profiles of Drosophila metastatic cultured tumors |
|
