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| Status |
Public on Apr 30, 2019 |
| Title |
Genome wide gene expression profiles of Drosophila metastatic cultured tumors |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Clones in the intestinal adult stem cell population mutant for Apc and Apc2, and over-expressing RasV12 and Snail are able to disseminate outside the gut and form macrometastases. These macrometastases can be cultured in allografts. This data shows the changes in gene expression profile of culturde tumors after 1, 2 and 10 (T1, T2, T10) rounds of allograft transplantation
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| Overall design |
We induced clones in adult Drosophila guts mutant for Apc and Apc2 and over-expressing UASRasV12, UASSnail and UASGFP under the Esg-Gal4 driver. We selected 3 macrometastases located at the abodmen, outside the gut, from 3 different flies, and performed an allograft culture in adult flies. We obtained a sample for each allograft at the first (T1), secod (T2) and tenth (T10) round of allograft cultire.
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| Contributor(s) |
Campbell K, Casali A |
| Citation(s) |
31127094 |
| |
| Submission date |
Jan 18, 2019 |
| Last update date |
Jun 18, 2019 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
[email protected]
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| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
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| Department |
A8G2 Group - Rheumatology Service
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| Street address |
Parc del Taulí, 1
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| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (9)
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| GSM3568797 |
metastasis sample from intestinal tumor reinjected specimen - T1 [2336 2016] |
| GSM3568798 |
metastasis sample from intestinal tumor reinjected specimen - T2 [2337 2016] |
| GSM3568799 |
metastasis sample from intestinal tumor reinjected specimen - T10 [2338 2016] |
| GSM3568800 |
metastasis sample from intestinal tumor reinjected specimen - T1 [2339 2016] |
| GSM3568801 |
metastasis sample from intestinal tumor reinjected specimen - T2 [2340 2016] |
| GSM3568802 |
metastasis sample from intestinal tumor reinjected specimen - T10 [2341 2016] |
| GSM3568803 |
metastasis sample from intestinal tumor reinjected specimen - T1 [2342 2016] |
| GSM3568804 |
metastasis sample from intestinal tumor reinjected specimen - T2 [2343 2016] |
| GSM3568805 |
metastasis sample from intestinal tumor reinjected specimen - T10 [2344 2016] |
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| Relations |
| BioProject |
PRJNA515841 |
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