|
| Status |
Public on Jul 01, 2019 |
| Title |
LNCaP-RT_ADT_MTH007G02G02_01, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP-N1-RT_ADT_MTH007G02G02_01
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP (hormone-sensitive PCa cells)
tissue: prostate
protocol: ADT added 2h before radiation and tRNA extracted 4h after radiation
|
| Treatment protocol |
LNCaP and C4-2 cells maintained in 6 well plates for 24 hours. After 24 hours the cells were treated with either 10 uM Enzalutamide (celleckchem) or ADT (charcopl phenol free media) or the combination of both, 2 hours later, the cells were radiated (4Gy). 4 hours after radiation, tRNA was extracted
|
| Growth protocol |
LNCaP and C4-2 cells are cultured in RPMI media containing 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The toal RNA was isolated by the Rneasy Mini Kit (Qiagen) according to the manufactures' instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was quantified using a NanoDrop Spectrophotometer ND-1000 (NanoDrop Technologies, Inc.) and its integrity was assessed using a 2100 Bioanalyzer (Agilent Technologies). Sense-strand cDNA was synthesized from 100 ng of total RNA, and fragmentation and labeling were performed to produce ss-cDNA with the GeneChip® WT Terminal Labeling Kit according to manufacturer’s instructions (Thermo Fisher Scientific).
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| |
|
| Hybridization protocol |
After fragmentation and labeling, 2.8 µg DNA target was hybridized on Clariom™S HT, Human (Thermo Fisher Scientific) and process on the GeneTitan® Instrument (Thermo Fisher Scientific) for Hyb-Wash-Scan automated workflow. For hybridization on the Gene Titan we use the GeneTitan™ Hybridization, Wash, and Stain Kit for WT Array Plates.
|
| Scan protocol |
The imaging device in GeneTitan MC Instrument uses an external, high-intensity xenon lamp and dual excitation and emission filters to capture images from array plates for use in expression.
|
| Description |
ADT added 2h before radiation and tRNA extracted 4h after radiation
|
| Data processing |
The primary analysis was completed using the R/Bioconductor package. The data was normalized using the 'rma' method in the bioconductor package 'oligo'.
|
| |
|
| Submission date |
Feb 21, 2019 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Thierry Muanza |
| Organization name |
McGill Univeristy
|
| Street address |
3755 Chemin de la Côte-Sainte-Catherine,
|
| City |
Montreal |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL24324 |
| Series (1) |
| GSE126881 |
Identification of Radiosensitivity Gene Signatures Induced by Enzalutamide in Prostate cancer cells |
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