liver RNA taken from untreated, 3 months old TLR3-/-
Growth protocol
mice were self-breeded in SPF
Extracted molecule
total RNA
Extraction protocol
RNA was extracted using Tri-zol, according to protocol
Label
biotin
Label protocol
Affymetrix wp protocol
Hybridization protocol
Affymetrix wp protocol
Scan protocol
Affymetrix wp protocol
Description
total liver RNA taken from untreated, 3 months old, TLR3-/-, triplicate-3
Data processing
Array data were normalized by the RMA algorithm using Affymetrix Expression Console. Gene and Gene Ontology (GO) annotations were determined for each probe set according to the annotation files published in the Affymetrix website (www.affymetrix.com). Data was transformed to log 2 scale and a threshold of 5 was applied.