|
| Status |
Public on May 17, 2010 |
| Title |
R1 ES cells |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stem cells R1
|
| Organism |
Mus musculus |
| Characteristics |
cell line: embryonic stem cells R1
embryo stage: pre-implantation epiblast, inner cell mass (ICM)
|
| Growth protocol |
R1 and E14TG2a (ES cells), A4 and G3 (TS cells) and F3 and F4 (XEN cells) were cultured in the absence of feeder cells according to standard protocols (http://www.sickkids.ca/research/rossant/custom/stemCells.asp). To induce TS cell differentiation, 1 x 10E5 cells were plated on to each well of a 6-well plate in standard TS cell conditions for 24h, rinsed twice with PBS and cultured for six days in unconditioned TS cell media without FGF4 and heparin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was processed using the RNeasy Mini Kit (Qiagen) with on-column RNase-free DNase (Qiagen) digestion.
|
| Label |
biotin
|
| Label protocol |
According to standard Affymetrix protocol. This service was performed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada
|
| |
|
| Hybridization protocol |
For microarray analysis, 100ng of total RNA was amplified and hybridized to the Mouse Gene 1.0 ST array (Affymetrix). This service was performed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada
|
| Scan protocol |
According to standard Affymetrix protocol. This service was performed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada
|
| Description |
Gene expression of mouse R1 embryonic stem cells.
|
| Data processing |
Data was processed using Expression Console from Affymetrix using the PLIER algorithm.
|
| |
|
| Submission date |
Apr 02, 2009 |
| Last update date |
Jan 05, 2012 |
| Contact name |
Brian Joseph Cox |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toronto
|
| Department |
Physiology
|
| Lab |
Cox System Biology
|
| Street address |
1 King's College Circle, Rm 3360
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE15519 |
Expression and ChIP-seq analyses of embryonic stem cells, extraembryonic endoderm stem cells, and trophoblast stem cells |
| GSE34799 |
Stem cell lines of the early mouse embryo |
|
