|
| Status |
Public on Oct 02, 2009 |
| Title |
E-IAA neg_A36.4 |
| Sample type |
RNA |
| |
|
| Source name |
PLN E-IAA neg, 5 weeks old NOD mice
|
| Organism |
Mus musculus |
| Characteristics |
genotype: PLN E-IAA neg
age: 5 weeks old
animal: NOD mice
strain: Non Obese Diabetic mouse for Type 1 diabetes
|
| Treatment protocol |
NOD mice positive or negative for Insulin AutoAntibodies as tested between 3 and 5 weeks of age were used at 5 weeks to isolate pancreatic lymph nodes. Immediately after dissection PLN have been placed in RNALater (Quiagen) buffer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed by using Qiagen mini- RNA kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip MG_U74Av2_A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from PLN of NOD mice at 5 weeks
|
| Data processing |
Raw data were preprocessed using RMA method
|
| |
|
| Submission date |
Apr 07, 2009 |
| Last update date |
Oct 02, 2009 |
| Contact name |
Evie Melanitou |
| E-mail(s) |
[email protected]
|
| Phone |
+33 1 40 61 3299
|
| Fax |
+33 1 45 68 8332
|
| Organization name |
Institut Pasteur
|
| Department |
Parasitology & Mycology
|
| Lab |
Immunophysiology and intracellular parasitism
|
| Street address |
25 rue du Dr Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE15582 |
Over expression of mRNA for multiple genes including insulin in the PLN of NOD is associated with Islet Autoimmunity |
|
