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Sample GSM399375 Query DataSets for GSM399375
Status Public on May 02, 2009
Title T.4SP24int.Th#3
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8- TCRhi 24int
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4SP24int.Th#3, CD4 Single-Positive, Semimature
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.9251
10344622 205.8197
10344624 362.5023
10344633 831.8948
10344637 253.9214
10344653 17.0973
10344658 163.0263
10344674 13.1479
10344679 41.1131
10344707 196.4257
10344713 430.1592
10344723 248.4472
10344725 45.3004
10344741 2980.668
10344743 37.3464
10344750 542.469
10344772 33.9162
10344789 183.145
10344797 419.0425
10344799 193.5674

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399375_EA07068_52779_MoGene_T.4SP24int.Th_#3.cel.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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