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Sample GSM399378 Query DataSets for GSM399378
Status Public on May 02, 2009
Title T.4SP69+.Th#3
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8- TCRhi 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4SP69+.Th#3, CD4 Single-Positive, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE73864

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.0857
10344622 250.1578
10344624 291.2856
10344633 757.5819
10344637 179.9649
10344653 18.361
10344658 124.5826
10344674 11.6469
10344679 38.1661
10344707 179.1413
10344713 375.2262
10344723 166.2454
10344725 50.9769
10344741 2774.39
10344743 21.6196
10344750 399.5211
10344772 23.767
10344789 96.8963
10344797 351.4887
10344799 162.8072

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399378_EA07068_52770_MoGene_T.4SP69+.Th_#3.cel.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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