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Sample GSM399383 Query DataSets for GSM399383
Status Public on May 02, 2009
Title T.8SP24int.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ TCRhi 24int
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8SP24int.Th#2, CD8 Single-Positive, Semimature
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.851
10344622 288.5913
10344624 441.7016
10344633 863.8127
10344637 266.2769
10344653 17.938
10344658 287.9339
10344674 9.8037
10344679 38.2258
10344707 277.1192
10344713 380.551
10344723 234.9921
10344725 50.0388
10344741 3702.7552
10344743 30.3998
10344750 535.6661
10344772 23.098
10344789 251.404
10344797 287.5898
10344799 253.1746

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399383_EA07068_52784_MoGene_T.8SP24int.Th_#2.cel.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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