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Sample GSM399384 Query DataSets for GSM399384
Status Public on May 02, 2009
Title T.8SP24int.Th#3
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ TCRhi 24int
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8SP24int.Th#3, CD8 Single-Positive, Semimature
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.4641
10344622 226.1684
10344624 461.791
10344633 965.8358
10344637 343.7134
10344653 15.0521
10344658 340.5748
10344674 9.7953
10344679 32.6222
10344707 326.3394
10344713 491.6911
10344723 349.2537
10344725 44.0181
10344741 4305.9615
10344743 23.8214
10344750 649.2071
10344772 20.6613
10344789 256.0432
10344797 202.2073
10344799 267.3357

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399384_EA07068_52785_MoGene_T.8SP24int.Th_#3.cel.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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