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Sample GSM399386 Query DataSets for GSM399386
Status Public on May 02, 2009
Title T.8SP69+.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ TCRhi 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8SP69+.Th#2, CD8 Single-Positive, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE73864

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.0553
10344622 237.4711
10344624 458.6068
10344633 696.2283
10344637 244.1141
10344653 12.7075
10344658 158.6977
10344674 12.7749
10344679 45.7862
10344707 227.9056
10344713 423.9353
10344723 248.1687
10344725 34.7162
10344741 2720.2149
10344743 27.8625
10344750 543.975
10344772 31.6226
10344789 185.028
10344797 354.8669
10344799 179.154

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399386_EA07068_52772_MoGene_T.8SP69+.Th_#2.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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