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Sample GSM399398 Query DataSets for GSM399398
Status Public on May 02, 2009
Title T.DPbl.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8+ TCR-/lo FSChi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.DPbl.Th#2, Double-Positive, Blasts
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.5793
10344622 310.9208
10344624 451.1618
10344633 831.4973
10344637 201.5011
10344653 18.0522
10344658 241.3951
10344674 9.8897
10344679 42.8598
10344707 212.6045
10344713 811.8163
10344723 173.5678
10344725 36.3815
10344741 5336.1701
10344743 31.1355
10344750 304.9018
10344772 26.2154
10344789 427.028
10344797 308.6412
10344799 449.3046

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399398_EA07068_56646_MoGene_T.DPbl.Th_#2.cel.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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