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Sample GSM399399 Query DataSets for GSM399399
Status Public on May 02, 2009
Title T.DPbl.Th#3
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8+ TCR-/lo FSChi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.DPbl.Th#3, Double-Positive, Blasts
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.0016
10344622 260.2854
10344624 397.5269
10344633 772.2163
10344637 175.6262
10344653 13.3197
10344658 327.2114
10344674 8.2543
10344679 35.9197
10344707 242.4796
10344713 715.7593
10344723 153.7698
10344725 34.8328
10344741 5230.5051
10344743 23.7745
10344750 287.1062
10344772 25.3327
10344789 478.2423
10344797 267.99
10344799 573.4287

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399399_EA07068_56647_MoGene_T.DPbl.Th_#3.cel.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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