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Sample GSM399441 Query DataSets for GSM399441
Status Public on May 02, 2009
Title B.FrF.BM#3
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics age: 8 weeks
exclusion markers: CD3 Ly6c Ter119 Gr1 Mac1
genetic background: C57BL/6J
phenotype markers: CD19+ IgM+ AA4.1- HSA+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol disruption by 1ml syringe, ACK lysis of RBCs
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description B.FrF.BM#3, Fr. F (recirc B)
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.8517
10344622 198.7021
10344624 307.66
10344633 820.683
10344637 173.7818
10344653 14.0085
10344658 303.538
10344674 12.3003
10344679 36.4936
10344707 400.5463
10344713 354.8477
10344723 179.8325
10344725 42.0904
10344741 2435.4994
10344743 28.1522
10344750 515.9292
10344772 29.9867
10344789 222.8456
10344797 305.4877
10344799 254.6515

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399441_EA07068_56614_MoGene_B.FrF.BM_#3.cel.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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