|
| Status |
Public on May 02, 2009 |
| Title |
SC.STSL.BM#1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
age: 10 weeks
exclusion markers: Ly6c Ter119 CD11b CD3 GR1 IgM CD24
genetic background: C57BL/6J
phenotype markers: CD117+ IL7R- CD150- CD48- AA4.1+ CD43+
separation method: dissection/trimming
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
disruption by 1ml syringe, ACK lysis of RBCs
|
| Label |
Biotin
|
| Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
| |
|
| Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
SC.STSL.BM#1, short-term reconstituting stem cell
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Apr 30, 2009 |
| Last update date |
May 24, 2016 |
| Contact name |
Liang Yang |
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE46349 |
