|
| Status |
Public on May 09, 2009 |
| Title |
Blood sample from patient 135 at Day 90 |
| Sample type |
RNA |
| |
|
| Source name |
Venous blood from healthy individual
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood
patient identifier: 135
day: Day 90
age (years): 58
sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the PAXgene 96 Blood RNA kit (Qiagen; Hilden, Germany) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
One microgram of total RNA from each blood sample was used to generate biotinylated cRNA, using the Agilent Low RNA Input Linear Amplification procedure (Version 2), with minor modifications (Agilent Technologies GmbH; Waldbronn, Germany).
|
| |
|
| Hybridization protocol |
The cRNA samples were hybridized overnight to Affymetrix U133 Plus 2.0 full genome oligonucleotide arrays and then stained with Streptavidin-Phycoerythrin according to the manufacturer’s instructions (Affymetrix Inc, CA, USA).
|
| Scan protocol |
Arrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from venous blood drawn in the morning from fasted subject.
|
| Data processing |
Gene signal intensities were computed using the MAS 5.0 algorithm (component of GCOS 1.0 software) using Affymetrix default analysis settings. Signal intensities were normalized using a quantile-quantile method [Bolstad, 2003].
|
| |
|
| Submission date |
May 08, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Guillemette Duchateau-Nguyen |
| E-mail(s) |
[email protected]
|
| Organization name |
F.Hoffmann-La Roche AG
|
| Department |
Pharma Research and Early Development
|
| Lab |
Translational Technologies and Bioinformatics
|
| Street address |
Building 93
|
| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16028 |
Longitudinal study of gene expression in healthy individuals |
|
| Relations |
| Reanalyzed by |
GSE119087 |
