|
| Status |
Public on Sep 30, 2020 |
| Title |
gcn20 versus Col7 replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Col7
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: wildtype Col7
age: two-weeks old seedlings
|
| Growth protocol |
Arabidopsis plants were grown on MS plates for 20 days after sowing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen RNA extraction kit following manufacturer's instructions
|
| Label |
cy3
|
| Label protocol |
Sample RNA (0.5 μg) was amplified and labeled with the Agilent Low Input Quick Amp Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
gcn20
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: gcn20 mutant
age: two-weeks old seedlings
|
| Growth protocol |
Arabidopsis plants were grown on MS plates for 20 days after sowing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen RNA extraction kit following manufacturer's instructions
|
| Label |
cy5
|
| Label protocol |
Sample RNA (0.5 μg) was amplified and labeled with the Agilent Low Input Quick Amp Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
Hybridization and slide washing were performed with the Gene Expression Hybridization Kit and Gene Expression Wash Buffers, respectively.
|
| Scan protocol |
slides were scanned in a GenePix 4000B microarray scanner, at 5 μm resolution and using the double scanning
|
| Data processing |
Feature Extraction software 9.5.1. was used for background substraction and normalization
|
| |
|
| Submission date |
Sep 03, 2019 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Jose Gadea |
| E-mail(s) |
[email protected]
|
| Organization name |
IBMCP
|
| Department |
Stress
|
| Street address |
Ingeniero Fausto Elio
|
| City |
Valencia |
| ZIP/Postal code |
46022 |
| Country |
Spain |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE136779 |
Arabidopsis seedlings: wild-type vs. gcn20 |
|
