|
| Status |
Public on Dec 01, 2009 |
| Title |
MEFs mTERT KO G1 3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: male
genotype: mTERT KO
generation: G1
cell type: embryonic fibroblasts
developmental stage: E12.5-14.5
passage number: 5
|
| Biomaterial provider |
Greider Lab, Sofia Vidal-Cardenas
|
| Treatment protocol |
none
|
| Growth protocol |
DMEM + 10% FBS + 1X PSG + 1X Normocin
37C, 5% CO2
passaged every 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction with Trizol reagent
clean-up with RNeasy kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter random primers for cDNA synthesis, and Invitrogen SuperScript II reverse transcriptase (www.affymetrix.com, GeneChip Expression Analysis, Technical Manual, 2003).
|
| |
|
| Hybridization protocol |
16hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual, 2004.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual, 2004.
|
| Description |
Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
|
| Data processing |
Image analysis and processing used GCOS 1.4 with the Expression Console PLIER algorithm and the manufacturer’s specifications.
|
| |
|
| Submission date |
Jun 03, 2009 |
| Last update date |
Dec 01, 2009 |
| Contact name |
Carol Greider |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Molecular Biology and Genetics
|
| Lab |
PCTB 605
|
| Street address |
725 N Wolfe St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE16430 |
Comparing effects of mTR and mTERT deletion on gene expression and DNA damage response: MEF |
| GSE16731 |
Comparing effects of mTR and mTERT deletion on gene expression and DNA damage response |
|
