Biotinylated cRNA were prepared according to the standard Affymetrix protocol for the Mouse 1.0ST arrays from 100ng total RNA.
Hybridization protocol
As per standard Affymetrix protocol for the Mouse 1.0ST arrays.
Scan protocol
Arrays were scanned on an DNA microarray Agilent Scanner
Description
RAW 264.7 transfected with control vector
Data processing
Quantile normalisation and Robust Multichip Average (RMA) background correction adjusting for probe sequence (Partek Software) was used to generate gene expression levels from the Mouse Gene 1.0ST arrays.
