|
| Status |
Public on Jul 09, 2009 |
| Title |
MEF_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblast cultured in vitro at passage less than 3 which were used to derive iPS cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
following manufacturer’s recommendation using Trizol reagent (Invitrogen)
WT cDNA Synthesis and Amplification Kit(Affymetrix, Inc.)
|
| Label |
biotin
|
| Label protocol |
WT Terminal Labeling Kit (Affymetrix, Inc.)
|
| |
|
| Hybridization protocol |
Hybridization, Wash and Stain Kit(Affymetrix, Inc.)
|
| Scan protocol |
GeneChip® Scanner 3000 7G3000 (Affymetrix, Inc.)
|
| Description |
no
|
| Data processing |
GCOS 1.4 software provided by Affymetrix
|
| |
|
| Submission date |
Jul 08, 2009 |
| Last update date |
Jul 08, 2009 |
| Contact name |
Lan Kang |
| Organization name |
Dalian Medical University
|
| Department |
Institute of Cancer Stem Cell
|
| Street address |
9 West Section, Lvshun South Road
|
| City |
Dalian |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE17004 |
Inducible iPS Cells Support Full-term Development of Tetraploid Blastocyst–Complemented Embryos |
|
