|
| Status |
Public on Oct 18, 2022 |
| Title |
lung_tumor_myckras_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
lung adenocarcinoma
|
| Organism |
Mus musculus |
| Characteristics |
strain: CCSP-rtTA/TetO-MYC/TetO-KRASG12D
treatment: on doxycycline (MYC and KRAS activated)
|
| Treatment protocol |
Doxycycline was added to mice's drinking water at a concentration of 100 µg/mL.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent after tissue homogenization.
|
| Label |
biotin
|
| Label protocol |
Each RNA sample was amplified using the Ambion Illumina RNA amplification kit with biotin UTP labeling. The Ambion Illumina RNA amplification kit uses T7 oligo(dT) primer to generate single stranded cDNA followed by a second strand synthesis to generate double-stranded cDNA, which is then column purified. In vitro transcription was done to synthesize biotin-labeled cRNA using T7 RNA polymerase. The cRNA was then column purified and measured.
|
| |
|
| Hybridization protocol |
A total of 1500 ng of cRNA was hybridized for each array using standard Illumina protocols with streptavidin-Cy3 being used for detection.
|
| Scan protocol |
Slides were scanned on an Illumina BeadArray reader and analyzed using BeadStudio (Illumina, Inc).
|
| Description |
Date: 2009
|
| Data processing |
The R package, lumi, computed normalized (log2-transformed, quantile-normalized) signal intensity valuees.
|
| |
|
| Submission date |
Jan 07, 2020 |
| Last update date |
Oct 18, 2022 |
| Contact name |
Dean Felsher |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Department |
Medicine
|
| Lab |
Felsher Lab
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE143253 |
Gene expression profiling of MYC inducible murine tumor models (tissues) [microarray] |
| GSE143254 |
Gene expression profiling of MYC inducible murine tumor models |
|
