|
| Status |
Public on Jan 04, 2021 |
| Title |
A3-FL-AM-like technical replicate 7 |
| Sample type |
SRA |
| |
|
| Source name |
AM-like
|
| Organism |
Sus scrofa |
| Characteristics |
strain: Large White
cell type: AM-like
infected: yes
virus: FL
original name (to remove): inf-AM-like_3
animal id: A3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from sorted cells was extracted using the Arcturus PicoPure RNA Isolation kit (ThermoFisher Scientific, St Aubin, France) according to the manufacturer’s instructions. The RNase-Free DNase Set (Qiagen, France) was used to remove contaminating DNA. RNA integrity was assessed using an RNA 6000 Pico kit on a Bioanalyzer 2100 (Agilent Technologies).
1 to 3 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Afterwards 150 pg of amplified cDNA was used to prepare library following Nextera XT DNA kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
AM-like, FL, Animal A3, technical replicate 7
|
| Data processing |
Illumina bcl2fastq 2.18.0.12 software used for demultiplexing.
tab-delimited text files include gene counting for each sample
Genome_build: ss102 (Ensembl 91)
Supplementary_files_format_and_content: reads counts
|
| |
|
| Submission date |
Mar 27, 2020 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Stephane Le Crom |
| Organization name |
IBENS
|
| Department |
Genomic Core Facility
|
| Street address |
46 rue Ulm
|
| City |
PARIS |
| ZIP/Postal code |
75230 |
| Country |
France |
| |
|
| Platform ID |
GPL20983 |
| Series (1) |
| GSE147632 |
Distinctive cellular and metabolic reprogramming in porcine lung myeloid cells infected with type I PRRSV strains |
|
| Relations |
| BioSample |
SAMN14468481 |
| SRA |
SRX8012285 |
