|
| Status |
Public on Sep 03, 2009 |
| Title |
JLMy82-1s microarray |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Chromosomal DNA from the experimental strain labeled with Cy5
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: JLMy82-1s
ploidy: Diploid
isogenicity: Isogenic to MS71
generations subcultured: 100
generations subcultured: 0
|
| Treatment protocol |
Strains were not treated with exogenous chemicals, etc.
|
| Growth protocol |
All strains were grown at 30 degrees Celcius for the number of generations indicated.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was harvested with a standard yeast DNA extraction protocol.
|
| Label |
Cy5
|
| Label protocol |
Direct incorporation with klenow polymerase and random priming.
|
| |
|
| Channel 2 |
| Source name |
Chromosomal DNA from MS71 labeled with Cy3
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: MS71
ploidy: Haploid
|
| Treatment protocol |
Strains were not treated with exogenous chemicals, etc.
|
| Growth protocol |
All strains were grown at 30 degrees Celcius for the number of generations indicated.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was harvested with a standard yeast DNA extraction protocol.
|
| Label |
Cy3
|
| Label protocol |
Direct incorporation with klenow polymerase and random priming.
|
| |
|
| |
| Hybridization protocol |
Described in Lemoine, F. et al. Cell, Vol. 120, 587-598 (2005).
|
| Scan protocol |
Array scanned in Genepix 4000b scanner.
|
| Description |
JLMy82-1s microarray
|
| Data processing |
The log2 R/G ratios for all probes in the array were collapsed to a single value in the case of repeated probes and normalized according to the median value for the entire array.
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Sep 02, 2009 |
| Contact name |
Jennifer Lynn McCulley |
| E-mail(s) |
[email protected]
|
| Phone |
919-684-5814
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Thomas Petes
|
| Street address |
374 CARL Building, Research Drive Box 3054
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL4414 |
| Series (1) |
| GSE17903 |
Elevated Levels of Aneuploidy and Chromosome Rearrangements are Separable Genome Instability Events |
|
