|
| Status |
Public on Jul 07, 2010 |
| Title |
Oneut-2 knockout duodenum, day 30, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse duodenum, Onecut-2 knockout, day 30
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Duodenum
genotype: Onecut-2 knockout
strain: 129
age: Postnatal day 30
gender: Mixed
|
| Treatment protocol |
No treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5.5 ug total RNA .
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.5 ug of cRNA were hybridized for 18 hr at 45C on Affymetrix Mouse Gene 1.0 ST Array in GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (protocol FS450-0007). Stained with R-Phycoerythrin Stepavidin (Molecular Probes) and probed with anti-streptavidin biotinylated goat antibody (Vector Laboratories).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G and GeneChip Operating Software.
|
| Description |
Mice during postnatal developmental phase of intestine, day 30
|
| Data processing |
The CEL files were processed through the Affymetrix Expression Console software (ver. 1.1). These were RMA normalized and the intensity values were used in Genespring GX 7.3.1 to create the experiments.
|
| |
|
| Submission date |
Sep 11, 2009 |
| Last update date |
Jul 07, 2010 |
| Contact name |
Bruce J Aronow |
| E-mail(s) |
[email protected]
|
| Phone |
513-636-4865
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18074 |
Expression in Duodenum of Wild type and Onecut-2 knockout mice at postnatal days 15 and 30 |
|
