|
| Status |
Public on Jun 01, 2010 |
| Title |
Congenital Hypothyroidism (CH) Patient 1830 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
dna isolated from blood of a patient with ch. gender: female
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood of a patient with ch.
gender: female
|
| Treatment protocol |
Whole genome amplification by GenomePlex (Sigma).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by QiaAmp DNA Blood Mini Kit Protocol.
|
| Label |
Cy3
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| Channel 2 |
| Source name |
DNA isolated from the blood of a donorpool of 5 healthy females.
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood of a donor pool of 5 healthy females.
gender: female
|
| Treatment protocol |
sonicated
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by QiaAmp DNA Blood Mini Kit Protocol.
|
| Label |
Cy5
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| |
| Hybridization protocol |
Hybridization was done overnight at 42?C in a slide booster (Advalytix, Brunnthal, Germany). Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| Scan protocol |
Slides were scanned using an Agilent scanner . PMT settings: 100/100
|
| Description |
Array CGH analysis of a patient with Congenital Hypothyroidism.
|
| Data processing |
TIFF images were analysed by Genepix 5.0 (Axon Instruments, Union City, CA) and raw intensities (gpr files) were imported into CGHPRO (Chen at al.,2005). Background intensities were not substracted. Signal intensities were normalized by subgridd LOWESS. Aberrant ratios involving three or more neighbouring clones wereconsidered as genomic aberrations, unless they coincided with a known polymorphism as listed in the Database of Genomic Variants (version Dec 2005)
|
| |
|
| Submission date |
Sep 17, 2009 |
| Last update date |
Mar 22, 2010 |
| Contact name |
Reinhard Ullmann |
| E-mail(s) |
[email protected]
|
| Phone |
00493084131251
|
| Organization name |
MPIMG
|
| Department |
Human Molecular Genetics
|
| Lab |
Molecular Cytogenetics
|
| Street address |
Ihnestr.73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5114 |
| Series (1) |
| GSE18152 |
Screening for chromosomal aberrations by array CGH in 74 patients with congenital hypothyroidism |
|
