|
| Status |
Public on Jun 09, 2020 |
| Title |
AV_1 dpi Mouse 1 |
| Sample type |
RNA |
| |
|
| Source name |
sorted splenic pDCs
|
| Organism |
Mus musculus |
| Characteristics |
cell type: sorted splenic pDCs
treatment: 1 day-post MAYV infection, oral antibiotic-treated
|
| Treatment protocol |
Mice were treated with oral antibiotics prior to infection Mayaro virus. Splenic pDCs were isolated using SiglecH-APC, B220-BV711, and PDCA-1 FITC.
|
| Growth protocol |
Mice were maintained under SPF conditions in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health after approval by the Institutional Animal Care and Use Committee at the Washington University School of Medicine (Assurance number A3381-01).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from sorted pDCs using the MagMAX™ mirVana™ Total RNA Isolation Kit as per manufacturer’s instructions.
|
| Label |
NA
|
| Label protocol |
nCounter Barcodes
|
| |
|
| Hybridization protocol |
Hybridization with Nanostring codeset reagents, sample, and buffer for 12 hours
|
| Scan protocol |
Analysis on the nCounter MAX/FLEX system
|
| Description |
2901012652_06
|
| Data processing |
The gene expression data were normalized to a set of 6 internal positive and 8 internal negative controls and then 14 internal reference genes. Values above the threshold (mean + 2SD of the negative controls) were considered for analysis. Normalized log2-transformed values were analyzed and unbiased hierarchical clustering was used to generate heatmaps in nSolver analysis software 4.0.
|
| |
|
| Submission date |
Jun 08, 2020 |
| Last update date |
Jun 09, 2020 |
| Contact name |
Emma Suening Winkler |
| E-mail(s) |
[email protected]
|
| Phone |
8123451756
|
| Organization name |
Washington University in St. Louis
|
| Department |
DOM-ID
|
| Lab |
Michael Diamond
|
| Street address |
660 S. Euclid Ave, Campus Box 8051
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL19964 |
| Series (1) |
| GSE152043 |
A commensal Clostridium bacteria restricts alphavirus dissemination through a bile acid-type I IFN signaling axis |
|
