|
| Status |
Public on Oct 20, 2009 |
| Title |
PPAR WT_DEHP 1150_58 |
| Sample type |
RNA |
| |
|
| Source name |
liver, PPAR WT_DEHP 1150_58
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
age: 7.5 mo +/-2.5 weeks
genotype/variation: PPAR WT
treatment: DEHP
|
| Treatment protocol |
Wild-type and PPAR(alpha)-null mice were given four daily gavage doses of DEHP (1150 mg/kg/bw/day) in corn oil or corn oil alone.
|
| Growth protocol |
Food (LabDiet 5001, PMI Nutrition International, St. Louis, MO) and municipal tap water were provided ad libitum. Animal facilities were controlled for temperature (20°C–24°C) and relative humidity (40%–60%) and kept under a 12-h light-dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated and purified from mouse livers according to the mirVana™ miRNA isolation kit (Ambion, Austin, TX) The integrity of each RNA sample was determined using an Agilent 2100 Bioanalyzer (Agilent, Foster City, CA), and RNA quantity was determined using a Nanodrop ND-1000 (Thermo Fisher Scientific, Wilmington, DE).
|
| Label |
biotin
|
| Label protocol |
Total RNA (300 ng per sample) was labeled using the Affymetrix GeneChip WT cDNA Synthesis and Amplification kit protocol.
|
| |
|
| Hybridization protocol |
following Affymetrix protocol
|
| Scan protocol |
The fluorescence intensities were measured with Affymetrix GeneChip scanner 3000 with 7G upgrade
|
| Description |
PPAR(alpha)-null mice (129S4/SvJae-Pparatm1Gonz/J, stock #003580) and wild-type mice (129S1/SvlmJ, stock #002448) were originally purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained as an inbred colony on the 129/Sv background
gene expression data from wildtype mouse liver treated with DEHP
|
| Data processing |
The intensity data files (.cel files) were loaded to Rosetta Resolver (Rosetta Biosoftware, Seattle, WA) for analysis.
|
| |
|
| Submission date |
Oct 14, 2009 |
| Last update date |
Oct 19, 2009 |
| Contact name |
Hongzu Ren |
| E-mail(s) |
[email protected]
|
| Phone |
919-541-4576
|
| Organization name |
US Environmental Protection Agency
|
| Lab |
NHEERL Toxicogenomics Core
|
| Street address |
109 TW Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18564 |
DEHP activation of PPAR(alpha) and CAR regulartory pathway in mouse liver |
|
