|
| Status |
Public on Oct 05, 2010 |
| Title |
CN2 |
| Sample type |
RNA |
| |
|
| Source name |
control normoxia
|
| Organism |
Mus musculus |
| Characteristics |
cell type: primary mouse microglia
genotype: wild-type
|
| Biomaterial provider |
The University of Washington (Seattle, WA).
|
| Treatment protocol |
Primary mouse microglia from WT and TLR4 knockout WT and TLR4 knockout were exposed to hypoxic or hypoglycemic (ischemic) or normoxic or normoglycemic (control) conditions for 24 h in triplicate. All cells were then exposed to 24 h of normoxic or normoglycemic (reperfusion) conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cultured myocytes by extraction with a commercially available kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix GeneChip® Mouse Gene 1.0 ST Arrays through a core facility in the Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
|
| Description |
primary mouse microglia
|
| Data processing |
Affymetrix Expression Console v1.1 RMA normalization log2
|
| |
|
| Submission date |
Oct 16, 2009 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18602 |
Microglia in ischemic brain injury |
|
