|
| Status |
Public on Oct 23, 2009 |
| Title |
R1 ES cells, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
R1 ES cells cultued in vitro
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
|
| Treatment protocol |
We change culture medium for these kinds of cells everyday; and all the manipulations were the same.
|
| Growth protocol |
P19 cells, P19 cells derived pluripotent stem cells and R1 ES cell were all cultured in DMED containing 15%FBS, nonessential amino acids, 2-mercaptoethanol, and 1000 units/ml leukemia inhibitory factor (LIF). All cells were cultured in incubator containing 5% CO2 at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
Gene expression data from ES cells
|
| Data processing |
The raw data was analyzed by R/BioConductor software and then output for further analysis. RMA was used to normalize the data with scale median=500. Then one way ANOVA (Matlab 7.5 )was used to screen out the differentially expressed genes.
|
| |
|
| Submission date |
Oct 22, 2009 |
| Last update date |
Oct 22, 2009 |
| Contact name |
Vetto Chang |
| Organization name |
NIBS
|
| Street address |
No. 7, Science Park Road, Zhongguancun Life Science Park, Beijing
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18691 |
Expression data of embryonal carcinoma (EC) cells derived pluripotent stem cells |
|
