|
| Status |
Public on Jul 07, 2021 |
| Title |
CTRL_Sultana_rep2 [OXNAC33] |
| Sample type |
RNA |
| |
|
| Source name |
Control leaves of Vitis vinifera cv. Sultana
|
| Organism |
Vitis vinifera |
| Characteristics |
cultivar: cv Sultana
genotype/variation: empty pK7WG2 containing a non-coding sequence
tissue: Young leaves
|
| Treatment protocol |
For transient transformation of Vitis vinifera cv. Sultana, the 35S:VviNAC33 construct was transferred to Agrobacterium tumefaciens strain C58C1 by electroporation. As control, Agrobacterium was also transformed with an empty pK7WG2 containing a non-coding sequence. Eight in vitro six-week-old plants of grapevine cv. Sultana for overexpression and seven for the control were immersed in each bacterial suspension and vacuum infiltrated (2 X 2 min at 90kPa).
|
| Growth protocol |
For the transient overexpression of VviNAC33 in grapevine, plantlets of Vitis vinifera cv. Sultana were in vitro micropropagated and cultivated in growth chamber at 25°C with a 16-h photoperiod.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from ~100 mg of the ground grapevine leaves tissue using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich).
|
| Label |
Cy3
|
| Label protocol |
For microarray analysis on Sultana leaves transiently overexpressing VviNAC33, the cDNA synthesis and labeling reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| |
|
| Hybridization protocol |
For microarray analysis on Sultana leaves transiently overexpressing VviNAC33, the hybridization and washing reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| Scan protocol |
Scanning and Feature Extraction were performed by using an Agilent Scanner following the settings and parameters indicated in the instruction manual.
|
| Data processing |
A data-matrix was prepared selecting from each single sub-array outcome file the gProcessedSignalvalues, which are the raw fluorescence intensities of each probe. The data were normalized on the 75th percentile and a correlation analysis was then performed to assess the consistency of the biological quadruplicates.
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| |
|
| Submission date |
Jul 24, 2020 |
| Last update date |
Jul 07, 2021 |
| Contact name |
Sara Zenoni |
| Organization name |
Università degli Studi di Verona
|
| Street address |
Strada Le Grazie 15
|
| City |
Verona |
| ZIP/Postal code |
37134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22427 |
| Series (1) |
| GSE155037 |
The VviNAC33 acts a molecular switch by activating leaf degreening and repressing vegetative growth during the immature-to-mature phase transition in grapevine |
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