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Sample GSM476655 Query DataSets for GSM476655
Status Public on Dec 02, 2009
Title Tgd.vg2+24ahi.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 5 weeks
exclusion markers: CD4- CD8- NK1.1-
genetic background: C57BL/6J
phenotype markers: TCRd+ Vg2+ CD24+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Tissue Grinder, MACS depletion / Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Tgd.vg2+24ahi.Th#1, thymus TCRd+ Vg2+ CD24+
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 01, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 21.2235
10344622 202.9562
10344624 281.251
10344633 606.2483
10344637 190.6618
10344653 15.667
10344658 241.0282
10344674 10.9365
10344679 44.9843
10344707 245.8956
10344713 526.1608
10344723 166.1354
10344725 38.7025
10344741 3320.1198
10344743 21.6128
10344750 293.7953
10344772 28.71
10344789 159.9413
10344797 365.5794
10344799 219.7319

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM476655_EA07068_54199_MoGene_immTgd.vg2+.Th_#1.cel.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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