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Sample GSM476673 Query DataSets for GSM476673
Status Public on Dec 02, 2009
Title Tgd.Sp#2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: CD4- CD8- NK1.1-
genetic background: C57BL/6J
phenotype markers: TCRd+ CD3e+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Tissue Grinder CD4 & CD8 Dynal Magnetic Bead Depletion Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Tgd.Sp#2, Spleen TCRgamma-delta, all DN
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 01, 2009
Last update date Jun 07, 2018
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE115450

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.6224
10344622 233.2866
10344624 270.253
10344633 723.6457
10344637 215.1742
10344653 26.3668
10344658 110.9391
10344674 17.6049
10344679 64.9449
10344707 223.8916
10344713 179.6156
10344723 152.7004
10344725 67.008
10344741 1529.5563
10344743 33.6526
10344750 261.2773
10344772 35.9478
10344789 69.4847
10344797 875.0826
10344799 151.2224

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM476673_EA07068_87591_MoGene_TGD.SP#2.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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