|
| Status |
Public on Sep 01, 2010 |
| Title |
Wild type, left ventricle, 4 ctrl injection, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
left ventricle, 4 ctrl injection
|
| Organism |
Mus musculus |
| Characteristics |
time point: 4 days
agent: control
gender: male
genotype: Wild type
age: 9-13 weeks
tissue: left ventricle myocardium
|
| Treatment protocol |
Mice were anesthetized with isoflurane and sacrificed by cervical dislocation. Left ventricle was quickly excised and immediately frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with an RNeasy mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
According to standard Affymetrix protocol (FS450_00007).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 h at 450C in the hybridization oven at 60 rpm, as described by manufacturer (Affymetrix).
|
| Scan protocol |
Gene Chips were scanned using the Hewlett Packard GeneArray Scanner 3000 7G.
|
| Description |
left ventricle, 4 ctrl injection, rep2
|
| Data processing |
The data were preprocessed using the robust multi-array average (RMA) package in R with default settings, i.e. quantile normalization and RMA background correction
|
| |
|
| Submission date |
Dec 07, 2009 |
| Last update date |
May 30, 2010 |
| Contact name |
Karina Hougen |
| Organization name |
University of Oslo
|
| Street address |
Kirkeveien 166
|
| City |
Oslo |
| ZIP/Postal code |
0407 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE19344 |
Expression data from tamoxifen treated and control injection treated Tg(αMHC-MerCreMer) and wild type mus musculus. |
|
