|
| Status |
Public on Oct 24, 2020 |
| Title |
OCI-LY10 50µM IRAKi 6h |
| Sample type |
RNA |
| |
|
| Source name |
OCI-LY10 50µM IRAKi 6h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: OCI-LY10
treatment: IRAKi
time: 6h
|
| Treatment protocol |
ABC-DLBCL cells were treated with 50µM IRAKI for 6h +/- CPI203 500nM for 18h
|
| Growth protocol |
ABC-DLBCL cells at exponential phase were set at 1x10e6 cells/ml for the experiment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA
|
| |
|
| Hybridization protocol |
RNA samples were processed to Affymetrix GeneChip HT HG-U219 arrays.
|
| Scan protocol |
Scanning was processed in the Gene Titan instrument.
|
| Description |
OCI-LY10 IRAKi
|
| Data processing |
Determination of the signal value for each probe set of the array were obtained with GeneChip® Command Console® Software (AGCC) (Affymetrix).
Raw data were normalized using the Robust Multichip Analysis (RMA) algorithm implemented in the Expression Console Software v1.1 (Affymetrix).
|
| |
|
| Submission date |
Oct 23, 2020 |
| Last update date |
Oct 24, 2020 |
| Contact name |
Gael Roue |
| E-mail(s) |
[email protected]
|
| Organization name |
Josep Carreras Leukaemia Research Institute
|
| Department |
Lymphoma Translational lab
|
| Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
|
| City |
Badalona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE159915 |
IRAK1/4 and BET bromodomain inhibitions converge on NF-κB blockade and display synergistic antitumor activity in ABC-DLBCL with MYD88L265P mutation |
|
