|
| Status |
Public on Jan 01, 2021 |
| Title |
SG142_11 |
| Sample type |
SRA |
| |
|
| Source name |
iCD103+ DC
|
| Organism |
Mus musculus |
| Characteristics |
treatment 2: γHV-68 (24 hpi)
treatment 3: 200 μM kynurenine
|
| Treatment protocol |
16 days post isolation cells were treated with γHV-68 (MOI of 1.0) w/ or w/o 200 μM kyn for 24 hours
|
| Growth protocol |
bone marrow stem cells were harvested from the femurs of C57Bl/6j mice and grown in RPMI suplemented with 10% FBS, 50 μM 2-βMercaptoethanol, 5 ng / mL murine GMCSF and 200 ng / mL murine FLT ligand. Medium was changed at 4 days and suspension cells were replated into fresh plates at 9 days. 16 days post isolation DCs were used for downstream experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RNAeasy columns
libraries and downstream sequencing was performed by genewiz
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
libraries and downstream sequencing was performed by genewiz
Supplementary_files_format_and_content: Data was normalized in Python / R using Deseq2. Non-normalized and normalized data is supplied in .csv format and contains read information for all 12 samples along with official gene symbol information.
|
| |
|
| Submission date |
Oct 28, 2020 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Stephen J. Gurczynski |
| E-mail(s) |
[email protected]
|
| Phone |
734-647-9968
|
| Organization name |
University Of Michigan
|
| Department |
Internal Medicine
|
| Lab |
Moore
|
| Street address |
109 Zina Pitcher Pl, Room 4062
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE160307 |
Kynurenine treatment of herpesivrus stimulated iCD103 dendritic cells |
|
| Relations |
| BioSample |
SAMN16581431 |
| SRA |
SRX9388916 |
