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GEO accession:
Sample GSM508412
Query DataSets for GSM508412
Status
Public on Feb 11, 2010
Title
C2TA wild-type_1
Sample type
RNA
Source name
CD80+ medullary thymic epithelial cells from WT, 3 biological replicates
Organism
Mus musculus
Characteristics
gender: female
strain: Balb/c
tissue: thymus
cell type: CD80+ medullary thymic epithelial cells
age: 4-6 weeks
genotype/variation: C2TA wild type
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from thymic medullary epithelial cells using the miRNAeasy kit (Qiagen) according to the manufacturer´s recommendations.
Label
biotin
Label protocol
WT Expression Kit
Hybridization protocol
hybridization, washing, staining: WT Expression Kit
Scan protocol
hardware: Genechip Scanner 3000 7G: Software: Genechip Command Console Fluidics Console
Description
3 biological replicates.
Data processing
RMA normalization
Submission date
Feb 10, 2010
Last update date
Feb 10, 2010
Contact name
Maria Hinterberger
E-mail(s)
[email protected]
Organization name
LMU
Department
Institut für Immunologie
Lab
AG Klein
Street address
Goethestr. 31
City
Munich
ZIP/Postal code
80336
Country
Germany
Platform ID
GPL6246
Series (1)
GSE20276
Silencing of C2TA reveals the autonomous role of medullary thymic epithelial cells in central CD4 T cell tolerance
Data table header descriptions
ID_REF
VALUE
log2-transformed, RMA signal intensity
Data table
ID_REF
VALUE
10362111
6.593667
10370376
5.176625
10370397
9.595778
10370413
5.944406
10370422
4.380224
10378781
6.593667
10379987
6.590306
10381681
6.593667
10396110
6.593667
10432682
6.257659
10432852
6.831645
10432897
6.980384
10444223
7.156263
10444229
5.867634
10444236
9.087902
10444284
5.872978
10444291
9.842238
10450154
10.11516
10450161
10.27348
10456005
10.82599
Total number of rows:
35552
Table truncated, full table size
621 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM508412_S896_WT1.CEL.gz
3.1 Mb
(ftp)
(http)
CEL
Processed data included within Sample table
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