|
| Status |
Public on Oct 02, 2010 |
| Title |
submandibular gland knockout rep3A |
| Sample type |
RNA |
| |
|
| Source name |
mouse submandibular gland
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
age: 2 months
tissue: submandibular gland
genotype: Car6 -/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
|
| Label |
Biotin
|
| Label protocol |
Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
|
| |
|
| Hybridization protocol |
750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
|
| Scan protocol |
Chips were scanned with Illumina BeadArray Reader v.3.5.49.29917 (Factor=1.52, PMT=552, Filter=100%)
|
| Description |
n/a
|
| Data processing |
Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
|
| |
|
| Submission date |
Feb 19, 2010 |
| Last update date |
Oct 02, 2010 |
| Contact name |
Pei-wen Pan |
| Organization name |
Institute of Medical Technology, University of Tampere
|
| Street address |
Biokatu 6
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE20423 |
Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice |
|
