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Sample GSM5134245 Query DataSets for GSM5134245
Status Public on Mar 01, 2024
Title C2: SfB-ABCFPfA_mfLon-rep2
Sample type SRA
 
Source name Ecoli cells
Organism Escherichia coli
Characteristics strain: SfB-ABCFPfA
abbreviations in data: C2
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Bcl2fastq (v2.17.1.14) software for base calling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to E.coli BL21(DE3) whole genome using Bowtie v2.2.6.
Fragments Per Kilo bases per Million reads (FPKM) were calculated using a protocol from Mortazavi et al., Nature methods, 2008
Genome_build: E.coli BL21(DE3) (GenBank: CP001509.3)
Supplementary_files_format_and_content: Matrix table with raw gene counts and FPKM values for every gene and every sample
 
Submission date Mar 04, 2021
Last update date Mar 01, 2024
Contact name Hao Qi
Organization name Tianjin University
Street address No. 135 Yaguan Road, Haihe Educaton Park
City Tianjin
ZIP/Postal code 300350
Country China
 
Platform ID GPL25368
Series (1)
GSE168226 undetermined
Relations
BioSample SAMN18138978
SRA SRX10237499

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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