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Status |
Public on Mar 01, 2024 |
Title |
C2: SfB-ABCFPfA_mfLon-rep2 |
Sample type |
SRA |
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Source name |
Ecoli cells
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Organism |
Escherichia coli |
Characteristics |
strain: SfB-ABCFPfA abbreviations in data: C2
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
Bcl2fastq (v2.17.1.14) software for base calling Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to E.coli BL21(DE3) whole genome using Bowtie v2.2.6. Fragments Per Kilo bases per Million reads (FPKM) were calculated using a protocol from Mortazavi et al., Nature methods, 2008 Genome_build: E.coli BL21(DE3) (GenBank: CP001509.3) Supplementary_files_format_and_content: Matrix table with raw gene counts and FPKM values for every gene and every sample
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Submission date |
Mar 04, 2021 |
Last update date |
Mar 01, 2024 |
Contact name |
Hao Qi |
Organization name |
Tianjin University
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Street address |
No. 135 Yaguan Road, Haihe Educaton Park
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City |
Tianjin |
ZIP/Postal code |
300350 |
Country |
China |
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Platform ID |
GPL25368 |
Series (1) |
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Relations |
BioSample |
SAMN18138978 |
SRA |
SRX10237499 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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