|
| Status |
Public on Jun 14, 2022 |
| Title |
spinal cord, W-20 from CVB3-infected G85R mice-R#2 |
| Sample type |
RNA |
| |
|
| Source name |
whole brain homogenates
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: G85R
age: Week-20
infection: CVB3-infected
tissue: spinal cord
|
| Treatment protocol |
This study involved direct used of mice organs and hence, no treatment protocol were involved.
|
| Growth protocol |
This study involved direct used of mice organs and hence, no growth protocol were involved.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole brain and spinal cord were homogenized with steel beads and digested with proteinase K to remove protein residuals. Homogenates were then lysed with lysis buffer & subjected to RNA extraction.
|
| Label |
optical barcodes from Nanostring Technologies, Seattle, WA.
|
| Label protocol |
70uL of hybridization buffer was added to 70uL of Reporter Probe Set with optical barcodes (Nanostring Technologies, Seattle, WA) and 8uL of the mix was added to 5uL of 100ng RNA. 2 uL of Capture Probe set was added and the mixture was mixed by inversion.
|
| |
|
| Hybridization protocol |
Reporter-sample-capture mixtures were hybridized at 65C in a thermal cycler with the heated lid temperature set to 70C for 22 hrs. The hybridized mixture was loaded into Nanostring nCounter FLEX prep station (Nanostring Technologies, Seattle, WA) for robotic processing with high sensitivity detection setting.
|
| Scan protocol |
The cartridge the probe/RNA hybrids were hybridized on in the prep station were loaded onto Nanostring nCounter scanner (Nanostring Technologies, Seattle, WA) and a scan was performed at 555 FOVs per lane/sample.
|
| Description |
500pfu_G85R_R#2_Week-20 (spinal cord)
|
| Data processing |
Data processing were performed by using NanoString nCounter Advanced Analysis Module
|
| |
|
| Submission date |
Mar 08, 2021 |
| Last update date |
Jun 16, 2022 |
| Contact name |
Honglin Luo |
| E-mail(s) |
[email protected]
|
| Organization name |
University of British Columbia
|
| Street address |
Room 166, 1081 Burrard Street
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6Z1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19964 |
| Series (1) |
| GSE168491 |
Gene expression profile in mock or virus-infected SOD1-G85R transgenic mice, a mouse model of amyotrophic lateral sclerosis |
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