NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM524714 Query DataSets for GSM524714
Status Public on Mar 23, 2010
Title Tie2_E15.5_1
Sample type RNA
 
Source name small blood vessels
Organism Mus musculus
Characteristics gene reported: Tg(TIE2GFP)287Sato / MGI:3056937
strain: CD-1
developmental stage: 15.5 dpc
theiler stage: TS23
Treatment protocol pool size: 4-5 Embryonic Kidneys
Pooled sample: Yes
Dissection Method: trypsinization & fluorescent activated cell sorting
Tie2-GFP transgenic mice are time-mated. Pregnant Tie2-GFP transgenic mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses and placed in ice-cold PBS. Embryonic kidneys are incubated in the presence of 300µl of trypsin for 5 minutes at 37 ºC. Kidneys are then dissociated by titurating in the presence of 600µl of ice-cold 10%FBS/PBS. The kidneys are pelleted at 5000 rpm, 4 ºC for 5 minutes. The media is aspirates and the cell pellet is resuspended in 200µl of ice-cold 2%FBS/PBS. Filter cells through 70 micron mesh filter and proceed with FACS.
Extracted molecule total RNA
Extraction protocol Potter protocol: 'RNA purification'
Label Biotin
Label protocol The FL-Ovation cDNA Biotin Module V2 (NuGEN) is used to chemically modify fragments and label target sense cDNA.
 
Hybridization protocol Affymetrix standard protocol
Amount labeled target hybridization to array: 2.5µg
The arrays were washed and and stained using a Fluidics Station 450 (Affymetrix) utilizing the fluidics protocol FS450-0007.
Scan protocol Probe arrays were scanned using an Affymetrix GeneChip Scanner 3000 7G and Affymetrix scanning software Genechip Operating Software Version 1.4.
Description >> Amplification protocol <<
Target Amplified manufacturer/kit: WT-Ovation Pico RNA Amplification System (NuGEN)
Target Amplified protocol: Potter protocols
Rounds of amplification: 1
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:13556
Data processing Analysis method: Affymetrix Expression Console and GeneSpring 10.
 
Submission date Mar 22, 2010
Last update date Dec 30, 2010
Contact name GUDMAP Developers
E-mail(s) [email protected]
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL6246
Series (1)
GSE20991 Gene expression profiles of E15.5 endothelial cells isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID:38)

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2)

Data table
ID_REF VALUE
10338001 12.76593
10338002 6.07640
10338003 10.98880
10338004 9.92199
10338005 2.81267
10338006 3.12160
10338007 3.47496
10338008 4.25044
10338009 6.87932
10338010 2.83439
10338011 5.59627
10338012 2.97983
10338013 2.68070
10338014 2.72912
10338015 2.68070
10338016 6.66576
10338017 13.80558
10338018 6.37504
10338019 5.33596
10338020 7.10498

Total number of rows: 35557

Table truncated, full table size 592 Kbytes.




Supplementary file Size Download File type/resource
GSM524714.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap