|
| Status |
Public on Sep 02, 2022 |
| Title |
RNAseq_DN67_MED12_unstim |
| Sample type |
SRA |
| |
|
| Source name |
primary T cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: unstimulated
genotype: MED12-null
|
| Treatment protocol |
cells were stimulated with anti-idiotye antibody for 3 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fresh cells using the Qiagen Rneasy kit.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequencing libraries were prepared by Novogene (Sacramento, CA) and 150 bp paired end sequencing at a depth of 3 x 10e7 reads per sample was obtained using the Illumina NovaSeq6000 platform
FASTQ files were generated by Novogene.
Transcripts were quantified with Salmon
DESeq2 was used to generate TPM (transcripts per million)
Genome_build: hg38
Supplementary_files_format_and_content: Data transcripts per million summarized to gene level. Each column in the table is a sample. Each row is a gene.
|
| |
|
| Submission date |
May 11, 2021 |
| Last update date |
Sep 02, 2022 |
| Contact name |
Julia Belk |
| Organization name |
Stanford University
|
| Street address |
450 Serra Mall
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE174279 |
Genome-wide maps of chromatin state in human primary CAR-T cells [RNA-seq] |
| GSE174282 |
Genome-wide maps of chromatin state in human primary CAR-T cells |
|
| Relations |
| BioSample |
SAMN19109939 |
| SRA |
SRX10861352 |
