Cells were treated with ZDON, Boc-DON, CystamineA, and Control
Extracted molecule
total RNA
Extraction protocol
Q7 and Q111 have been treated with ZDON (50µM) or Boc-DON (50µM) or cystamine (250µM) for 12 hours. Cells were washed with cold PBS and RNA has been extracted with the RNAgents Total RNA Isolation System Kit (Promega), as directed by the manufacturer. Briefly, cells were lysed in chilled Denaturating Solution and the RNA was precipitated and isolated through Phenol:Chloroform:Isoamyl Alchool extraction.
Label
Biotin
Label protocol
Illumina protocol
Hybridization protocol
Illumina protocol
Scan protocol
Illumina protocol
Description
Q111 striatal cell line treated with ZDON 4068264040_G
