NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM538229 Query DataSets for GSM538229
Status Public on Apr 29, 2010
Title B1b.PC#2
Sample type RNA
 
Source name Peritoneal Cavity
Organism Mus musculus
Characteristics age: 7 weeks
exclusion markers: CD3 Ly6c Ter119 Gr-1 Mac1
genetic background: C57BL/6J
phenotype markers: IgD+ IgM+ CD19+ CD43+ CD5- CD45R+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Peritoneal cavity washout with 10ml of staining medium
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description B1b.PC#2, B-1b, peritoneal cavity
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.5216
10344622 302.0484
10344624 352.7167
10344633 624.5753
10344637 174.6267
10344653 17.1362
10344658 324.203
10344674 9.091
10344679 36.5885
10344707 1088.7846
10344713 404.6969
10344723 239.6951
10344725 37.5923
10344741 2561.7062
10344743 21.5803
10344750 777.7331
10344772 37.6718
10344789 225.2438
10344797 271.0194
10344799 307.156

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538229_EA07068_56619_MoGene_B1b.PC_#2.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap