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Sample GSM538281 Query DataSets for GSM538281
Status Public on Apr 29, 2010
Title DC.LC.Sk#5
Sample type RNA
 
Source name skin
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: CD103- CD3-
genetic background: C57BL/6J
phenotype markers: MHCII+ CD11b+ CD45+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Disruption using syringe
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description DC.LC.Sk#5, epidermal langerhans cells
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date Aug 13, 2019
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSM1832691
Reanalyzed by GSE74316
Reanalyzed by GSE135788

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 19.287
10344622 274.187
10344624 476.5294
10344633 894.3366
10344637 589.8162
10344653 15.6324
10344658 261.5681
10344674 8.7762
10344679 37.873
10344707 171.6456
10344713 732.5022
10344723 220.4852
10344725 47.9502
10344741 2021.2117
10344743 19.4069
10344750 661.0301
10344772 39.0283
10344789 170.0667
10344797 270.6245
10344799 180.5474

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM538281_EA07068_111376_MoGene_DC.LC.SK#5.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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