NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM538294 Query DataSets for GSM538294
Status Public on Apr 29, 2010
Title NK.DAP10-.Sp#1
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 7 weeks
exclusion markers: CD3 CD4 CD5 CD8 CD19 Gr-1 Ter119
genetic background: C57BL/6J
phenotype markers: NK1.1+ TCRb-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing, FACS sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description NK.DAP10-.Sp#1, Splenic NK cells from DAP10-/- mice
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 19.0712
10344622 245.1797
10344624 282.4339
10344633 681.4924
10344637 125.1609
10344653 19.7492
10344658 155.2911
10344674 10.2978
10344679 61.0441
10344707 229.9632
10344713 173.6115
10344723 192.8721
10344725 49.4162
10344741 2051.268
10344743 43.873
10344750 129.6
10344772 28.794
10344789 71.9409
10344797 690.9411
10344799 184.8817

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538294_EA07068_93784_MoGene_NK.DAP10-.SP#1.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap