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Sample GSM538313 Query DataSets for GSM538313
Status Public on Apr 29, 2010
Title NK.MCMV7.Sp#2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: CD3 CD4 CD5 CD8 CD19 Gr-1 Ter119
genetic background: C57BL/6J
phenotype markers: NK1.1+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing, FACS sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description NK.MCMV7.Sp#2, Splenic NK cells MCMV d7 PI
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 13.9879
10344622 252.3446
10344624 388.6663
10344633 753.4621
10344637 221.7591
10344653 18.0431
10344658 356.8391
10344674 9.9095
10344679 42.4068
10344707 397.2238
10344713 237.368
10344723 135.4139
10344725 45.6958
10344741 2753.3848
10344743 25.1787
10344750 140.5612
10344772 24.6678
10344789 204.4424
10344797 286.98
10344799 249.6543

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538313_EA07068_90293_MoGene_NK.MCMV7#2.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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