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Status
Public on Apr 29, 2010
Title
NKT.4+.Lv#3
Sample type
RNA
Source name
liver
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
homogenize, ficoll, stain double sort on FACS Aria
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.4+.Lv#3, CD4+ iNKT cells from Liver
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
19.6504
10344622
249.2702
10344624
370.1258
10344633
899.1536
10344637
217.0466
10344653
17.6133
10344658
212.9776
10344674
11.2987
10344679
46.5098
10344707
278.5847
10344713
407.1586
10344723
174.2354
10344725
47.5095
10344741
3343.8015
10344743
28.5567
10344750
186.4316
10344772
24.9353
10344789
193.1642
10344797
345.4408
10344799
292.3097
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538320_EA07068_91099_MoGene_NKT.4+.LV#3.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
