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Status
Public on Apr 29, 2010
Title
NKT.4+.Sp#2
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
homogenized, ACK lyszed, stained MACS enrichement, double sort on FACS Aria
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.4+.Sp#2, CD4+ iNKT cells from Spleen
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.0872
10344622
241.6741
10344624
317.5541
10344633
653.5431
10344637
158.4321
10344653
22.243
10344658
30.7331
10344674
13.0007
10344679
59.7567
10344707
56.3643
10344713
635.8102
10344723
221.1851
10344725
48.4685
10344741
1460.4116
10344743
36.9565
10344750
133.5672
10344772
25.5818
10344789
45.5526
10344797
640.3326
10344799
79.488
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538323_EA07068_91092_MoGene_NKT.4+.SP#2.CEL.gz
4.0 Mb
(ftp) (http) CEL
Processed data included within Sample table
