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Status
Public on Apr 29, 2010
Title
NKT.4+.Sp#3
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
homogenized, ACK lyszed, stained MACS enrichement, double sort on FACS Aria
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.4+.Sp#3, CD4+ iNKT cells from Spleen
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
17.3084
10344622
293.851
10344624
403.1621
10344633
823.6537
10344637
236.2139
10344653
17.77
10344658
123.3715
10344674
10.4095
10344679
42.6892
10344707
163.9367
10344713
521.9605
10344723
174.7917
10344725
37.6302
10344741
2720.2472
10344743
23.3865
10344750
246.5368
10344772
25.2012
10344789
147.2338
10344797
367.0791
10344799
157.9656
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538324_EA07068_91093_MoGene_NKT.4+.SP#3.CEL.gz
4.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
